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Further Reading on 'The Shogun's Queen' A voice to the voiceless: the challenges and rewards of writing fiction set in a distant place and time I wanted to write a novel set in 19th-century Japan, a time of enormous upheaval when the ruling shogun was toppled and a new westernising government installed in power. The Shogun's Harem by Lesley Downer. Our November Guest is Lesley Downer, who will become a regular History Girl next year. Lesley Downer lived in Japan for many years. She tramped around Basho's Narrow Road the Deep North, lived among geisha, interviewed sumo wrestlers and enjoyed the glitzy life of Tokyo. She is the author of many books on. Buy The Shogun's Queen by Lesley Downer from Waterstones today! Click and Collect from your local Waterstones or get FREE UK delivery on orders over £
Genome sequencing of virus has become a useful tool for better understanding of virus pathogenicity and epidemiological surveillance. Obtaining virus genome sequence directly from clinical samples is still a challenging task due to the low load of virus genetic material compared to the host DNA, and to the difficulty to get an accurate genome assembly. Here we introduce a complete sequencing and analyzing protocol called V-ASAP for Virus Amplicon Sequencing Assembly Pipeline. Our protocol is able to generate the viral dominant genome sequence starting from clinical samples. It is based on a multiplex PCR amplicon sequencing coupled with a reference-free analytical pipeline. This protocol was applied to 11 clinical samples infected with coronavirus OC43 (HcoV-OC43), and led to seven complete and two nearly complete genome assemblies. The protocol introduced here is shown to be robust, to produce a reliable sequence, and could be applied to other virus.
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